Specificity of a sandwich enzyme-linked immunosorbent assay for detecting Aspergillus galactomannan.
نویسندگان
چکیده
The specificity of a sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Aspergillus galactomannan was tested with exoantigens of 29 fungi cultured from clinical specimens. Cross-reactivity was observed with Penicillium chrysogenum, Penicillium digitatum, and Paecilomyces variotii. Furthermore, 40 serum samples obtained from bacteremic patients with hematologic malignancies were retrospectively tested by sandwich ELISA. False-positive reactions with the serum were reproducible but did not correspond with the results of culture of specific microorganisms. Moreover, the microorganisms cultured from the blood showed no reactivity by the sandwich ELISA.
منابع مشابه
Sandwich enzyme-linked immunosorbent assay compared with Pastorex latex agglutination test for diagnosing invasive aspergillosis in immunocompromised patients.
The performance of a direct sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Aspergillus galactomannan was compared with that of the Pastorex Aspergillus antigen latex agglutination (LA) test by using 532 serum samples from 61 patients at risk for invasive aspergillosis. The ELISA gave positive results earlier in the course of infection than did the LA test. A sensitivity of 70%...
متن کاملSignificance of galactomannan antigen for aspergillosis diagnosis: A review
Invasive aspergillosis is one the major causes of death in hematopoietic and solidtransplantrecipients. One of the most critical problems in medical mycology is theinability to consistently make a convincing and early diagnosis of invasive fungalinfection. Conventional diagnosis methods such as culture-based approaches are limitedby the insufficient accessibility sensitivity and the non-culture...
متن کاملSignificance of galactomannan antigen for aspergillosis diagnosis: A review
Invasive aspergillosis is one the major causes of death in hematopoietic and solidtransplant recipients. One of the most critical problems in medical mycology is the inability to consistently make a convincing and early diagnosis of invasive fungal infection. Conventional diagnosis methods such as culture-based approaches are limited by the insufficient accessibility sensitivity and the non-cul...
متن کاملCross-reactivity of Fusarium spp. in the Aspergillus Galactomannan enzyme-linked immunosorbent assay.
Nine of 11 hematological patients with disseminated/deep-seated Fusarium infection tested at least twice for Aspergillus galactomannan (GM) had repeated positive results in the absence of Aspergillus isolation in culture. The centrifuged supernatants of 12 Fusarium isolates were tested by a GM enzyme-linked immunosorbent assay (EIA). All the isolates produced positive reactions when tested undi...
متن کاملAspergillus galactomannan enzyme-linked immunosorbent assay cross-reactivity caused by invasive Geotrichum capitatum.
We report three cases of invasive Geotrichum capitatum infection in patients with acute leukemia for which an enzyme-linked immunosorbent assay (ELISA) for Aspergillus galactomannan was positive, with no evidence of aspergillosis. Supernatants obtained from suspensions of 17 G. capitatum strains gave positive reactions with the Aspergillus galactomannan ELISA. These clinical and laboratory data...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 35 1 شماره
صفحات -
تاریخ انتشار 1997